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STEMCELL Technologies ALDEFLUOR® kit
The ALDEFLUOR® reagent system is a non-immunological method to identify human stem/progenitor cells on the basis of their aldehyde dehydrogenase (ALDH) activity.
High ALDH expression has been reported for normal and cancer precursor cells of various lineages, including hematopoietic, mammary, endothelial, mesenchymal, and neural.
Cells expressing high levels of ALDH become brightly fluorescent (ALDHbr) and can be identified and enumerated using a standard flow cytometer or isolated by cell sorting for further purification and characterization.
The use of an internal cellular enzyme for identifying and isolating stem and progenitor cells provides an alternative to the more traditional method of staining with antibodies against cell surface antigens.
The ALDEFLUOR™ fluorescent reagent system has supported over 200 publications to date. This fast, antibody-free assay detects more than 80 types of stem, progenitor, and cancer precursor cells by measuring their activity levels of aldehyde dehydrogenase (ALDH) using a flow cytometer. This video guide demonstrates the correct use of the flow cytometer for analyzing ALDEFLUOR™-stained ALDH bright cells. Watch other videos here.