EXBIO Lineage Cocktail

Format / Formulation

The reagent contains premixed combination of mouse monoclonal antibodies against human CD3, CD14, CD16, CD19, CD20 and CD56 antigens (clone MEM 57, MEM-15, LNK16, LT19, LT20 and MEM-188, respectively) labeled with Fluorescein isothiocyanate (FITC). Labeled antibodies are diluted in an optimal concentration in phosphate buffered saline (PBS) containing 15mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent. The content of a vial (1 ml) is sufficient for 50 tests.

Fig. 1. Identification of dendritic cells in human peripheral blood by their lack of staining with the Lineage Cocktail (CD3 / CD14 / CD16 / CD19 / CD20 / CD56) FITC and positive staining with anti-HLA-DR antibody conjugated with PE. 100 000 events were acquired for identification of HLA-DR⁺ dendritic cells.

Fig. 2. Identification of basophils in human peripheral blood by their lack of staining with the Lineage Cocktail (CD3 / CD14 / CD16 / CD19 / CD20 / CD56) FITC and positive staining with anti-IgE antibody conjugated with PE-Dyomics 647. 60 000 events were acquired for identification of IgE⁺ basophils.