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Cytognos Kit for Paroxismal Nocturnal Hemoglobinuria
Screening Kit for Paroxismal Nocturnal Hemoglobinuria
Screening Kit for PNH is a four-color direct immunofluorescence reagent for use in flow cytometry to detect the existence of GPI deficient cells with a 100 % of sensitivity.
The kit includes the following antibody combination:
Analysis strategy of this combination: anti-CD45 and anti-CD64 antibodies allow the identification of the populations to be studied, neutrophils and monocytes. Then, anti-CD16 and anti-CD14 antibodies detect loss of expression of the CD16 molecule on neutrophils and the CD14 molecule on monocytes
Paroxismal Nocturnal Hemoglobinuria (PNH) is an acquired clonal hematopoietic stem-cell disorder related to the occurrence of a somatic mutation in the PIG-A gen, located in the human X chromosome. This genetic alteration results in a partial or total deficiency of all proteins normally linked to the cell membrane by a glycosylphosphatidylinositol (GPI) anchor (GPI-anchored proteins).
With this antibody combination, it is possible to detect the existence of GPI deficient cells with a 100% of sensitivity
